DBCO-PEG-SH，二苯并環(huán)辛烯PEG巰基

DBCO-PEG-SH，二苯并環(huán)辛烯PEG巰基，DBCO-PEG-Thiol，產(chǎn)品芃碩生物強(qiáng)勢(shì)推出，自主研發(fā)，歡迎選購(gòu)！

（備注：非常規(guī)分子量：400、600。精確聚合度的小分子定制。）

結(jié)構(gòu)式：

基本信息：

中文名稱：二苯并環(huán)辛烯-聚乙二醇-巰基 (DBCO-PEG-SH)

別名：DBCO-PEG-Thiol

英文名稱：DBCO-PEG-Thiol，DBCO-PEG-SH

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

純度：95%以上

DBCO-PEG-SH，二苯并環(huán)辛烯PEG巰基，DBCO-PEG-Thiol，產(chǎn)品芃碩生物強(qiáng)勢(shì)推出，自主研發(fā)，歡迎選購(gòu)！

應(yīng)用簡(jiǎn)介：

DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Copper-free Click Reaction Procedures:

Prepare the azide-containing reagents in reaction buffer.

Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4℃.

Purify conjugates either by dialysis or size-exclusion chromatograph.

物理性質(zhì)：

狀態(tài)：淡黃色到白色固體，或者半固體，取決于分子量。

溶解性：溶于水，二氯甲烷，DMSO,DMF等溶劑。

保持條件：-20℃*保存，避光，干燥。

注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干。

反應(yīng)性：DBCO于含有N3基團(tuán)的反應(yīng)。HS可以和MAL反應(yīng)，也可以修飾納米金。

DBCO-PEG-SH，二苯并環(huán)辛烯PEG巰基，DBCO-PEG-Thiol，產(chǎn)品芃碩生物強(qiáng)勢(shì)推出，自主研發(fā)，歡迎選購(gòu)！

技術(shù)指標(biāo)：

純度（PEG）：95%以上

取代率（DBCO）：90%以上

取代率（SH）：90%以上

分散系數(shù)（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF

相關(guān)產(chǎn)品*：

MPEG-DBCO

DBCO-PEG-NH2

DBCO-PEG-COOH

DBCO-PEG-NHS

DBCO-PEG-SH

DBCO-PEG-ACRL

DBCO-PEG-ACA

DBCO-PEG-DSPE

DBCO-PEG-FITC

DBCO-PEG-RB

DBCO-PEG-MAL

DBCO-PEG-FA

DBCO-PEG-BIOTIN

DBCO-PEG-OH

DBCO-PEG-OPSS

LA-PEG-DBCO

4ARM-PEG-DBCO

DBCO-PEG-SH，二苯并環(huán)辛烯PEG巰基，DBCO-PEG-Thiol，產(chǎn)品芃碩生物強(qiáng)勢(shì)推出，自主研發(fā)，歡迎選購(gòu)！