Microfluidizer Cell Disruption Application Charts

Sample

PSI

Passes

Results

Meningicoccal cell paste
(4%) in buffer

 1000

1

Cooling used to maintain 10C temperature. Complete rupture achieved in one pass.

Mammalian cells

 2000

1

Complete rupture of cells and easy separation of parasite within the cell achieved in one pass on laboratory model H-5000.

E. coli (10%) in buffer

6000

3

90% rupture achieved. Results scaled linearly from lab to production.

10000

1

90% rupture achieved.

10000

3

90% rupture achieved.

Baker's yeast

20000

10

Cooling used. 95% rupture of yeast cells achieved.

Brewer's yeast (10%) in water

10000

1-10

Cooling used to keep temp below 5C. 35% rupture at 1 pass and 63% in 10 passes.

20000

1-10

71% rupture at 1 pass and 98% in 10 passes. Yield of enzyme significantly better than that achieved with other processing techniques including French press.