只需 5 分鐘,ExoSAP-IT™ Express PCR Product Cleanup Reagent 即可從 PCR 反應中除去過量的引物和未結合的核苷酸。與替代純化方法(如離心柱或微珠)相比,該酶法純化方法是一種更精確、產量更高和周轉時間更快的方法。
• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 99% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup
ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. 反應設置僅需一個移液步驟即可完成,其后是兩次短暫孵育。次孵育可以水解過量的引物并使核苷酸去磷酸化。第二次高溫孵育在約一分鐘內不可逆地滅活酶。將 ExoSAP-IT Express 試劑直接加入 PCR 產物中,可省略向管、孔或柱的轉移步驟并節省 PCR 擴增子,從而有助于降低交叉污染的可能性;此外,也無需進一步處理。樣品即可用于 DNA 測序或 SNP 分析。
• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 99% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup
ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. 反應設置僅需一個移液步驟即可完成,其后是兩次短暫孵育。次孵育可以水解過量的引物并使核苷酸去磷酸化。第二次高溫孵育在約一分鐘內不可逆地滅活酶。將 ExoSAP-IT Express 試劑直接加入 PCR 產物中,可省略向管、孔或柱的轉移步驟并節省 PCR 擴增子,從而有助于降低交叉污染的可能性;此外,也無需進一步處理。樣品即可用于 DNA 測序或 SNP 分析。


















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